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Dr. Andrew Kaufman refutes 'isolation' of SARS-Cov-2; he does step-by-step analysis of a typical claim of isolation; there is no proof that the virus exists

by Jon Rappoport, April 21, 2021

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The global medical community has been asserting that "a pandemic is being caused by a virus, SARS-Cov-2."

But what if the virus doesn’t exist?

People have been asking me for a step-by-step analysis of a mainstream claim of virus-isolation. Well, here it is.

'Isolation' should mean the virus has been separated out from all surrounding material, so researchers can say, "Look, we have it. It exists."

I took a typical passage from a published study, a 'methods' section, in which researchers describe how they "isolated the virus." I sent it to Dr. Andrew Kaufman [1], and he provided his analysis in detail.

I found several studies that used very similar language in explaining how 'SARS-CoV-2 was isolated'. For example, Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States, (Emerging Infectious Diseases, Vol. 26, No. 6 — June 2020) [2].

First, I want to provide a bit of background that will help the reader understand what is going on in the study.

The researchers are creating a soup in the lab. This soup contains a number of compounds. The researchers assume, without evidence that 'the virus' is in this soup. At no time do they separate the purported virus from the surrounding material in the soup. Isolation of the virus is not occurring.

They set about showing that the monkey (and/or human cells) they put in the soup are dying. This cell-death, they claim, is being caused by 'the virus'. However, as you’ll see, Dr. Kaufman dismantles this claim.

There is no reason to infer that SARS-CoV-2 is in the soup at all, or that it is killing cells.

Finally, the researchers assert, with no proof or rational explanation, that they were able to discover the genetic sequence of 'the virus'.

Here are the study’s statements claiming isolation, alternated with Dr. Kaufman’s analysis:

STUDY: "We used Vero CCL-81 cells for isolation and initial passage…"

KAUFMAN: "Vero cells are foreign cells from the kidneys of monkeys and a source of contamination. Virus particles should be purified directly from clinical samples in order to prove the virus actually exists. Isolation means separation from everything else. So how can you separate/isolate a virus when you add it to something else?"

STUDY: "…We cultured Vero E6, Vero CCL-81, HUH 7.0, 293T, A549, and EFKB3 cells in Dulbecco minimal essential medium (DMEM) supplemented with heat-inactivated fetal bovine serum (5% or 10%)…"

KAUFMAN: "Why use minimal essential media, which provides incomplete nutrition [to the cells]? Fetal bovine serum is a source of foreign genetic material and extracellular vesicles, which are indistinguishable from viruses."

STUDY: "…We used both NP and OP swab specimens for virus isolation. For isolation, limiting dilution, and passage 1 of the virus, we pipetted 50 μL of serum-free DMEM into columns 2–12 of a 96-well tissue culture plate, then pipetted 100 μL of clinical specimens into column 1 and serially diluted 2-fold across the plate…"

KAUFMAN: "Once again, misuse of the word isolation."

STUDY: "…We then trypsinized and resuspended Vero cells in DMEM containing 10% fetal bovine serum, 2× penicillin/streptomycin, 2× antibiotics/antimycotics, and 2× amphotericin B at a concentration of 2.5 × 105 cells/mL…"

KAUFMAN: "Trypsin is a pancreatic enzyme that digests proteins. Wouldn’t that cause damage to the cells and particles in the culture which have proteins on their surfaces, including the so called spike protein?"

KAUFMAN: "Why are antibiotics added? Sterile technique is used for the culture. Bacteria may be easily filtered out of the clinical sample by commercially available filters (GIBCO) [3]. Finally, bacteria may be easily seen under the microscope and would be readily identified if they were contaminating the sample. The specific antibiotics used, streptomycin and amphotericin (aka ‘ampho-terrible’), are toxic to the kidneys and we are using kidney cells in this experiment! Also note they are used at ‘2X’ concentration, which appears to be twice the normal amount. These will certainly cause damage to the Vero cells."

STUDY: "…We added [not isolated] 100 μL of cell suspension directly to the clinical specimen dilutions and mixed gently by pipetting. We then grew the inoculated cultures in a humidified 37°C incubator in an atmosphere of 5% CO2 and observed for cytopathic effects (CPEs) daily. We used standard plaque assays for SARS-CoV-2, which were based on SARS-CoV and Middle East respiratory syndrome coronavirus (MERS-CoV) protocols…"

STUDY: "When CPEs were observed, we scraped cell monolayers with the back of a pipette tip…"

KAUFMAN: "There was no negative control experiment described. Control experiments are required for a valid interpretation of the results. Without that, how can we know if it was the toxic soup of antibiotics, minimal nutrition, and dying tissue from a sick person which caused the cellular damage or a phantom virus? A proper control would consist of the same exact experiment except that the clinical specimen should come from a person with illness unrelated to covid, such as cancer, since that would not contain a virus."

STUDY: "…We used 50 μL of viral lysate for total nucleic acid extraction for confirmatory testing and sequencing. We also used 50 μL of virus lysate to inoculate a well of a 90% confluent 24-well plate."

KAUFMAN: "How do you confirm something that was never previously shown to exist? What did you compare the genetic sequences to? How do you know the origin of the genetic material since it came from a cell culture containing material from humans and all their microflora, fetal cows, and monkeys?"

—end of study quotes and Kaufman analysis—

My comments: Dr. Kaufman does several things here. He shows that isolation, in any meaningful sense of the word 'isolation'. is not occurring.

Dr. Kaufman also shows that the researchers want to use damage to the cells and cell-death as proof that "the virus" is in the soup they are creating. In other words, the researchers are assuming that if the cells are dying, it must be the virus that is doing the killing. But Dr. Kaufman shows there are obvious other reasons for cell damage and death that have nothing to do with a virus. Therefore, no proof exists that 'the virus' is in the soup or exists at all.

And finally, Dr. Kaufman explains that the claim of genetic sequencing of "the virus" is absurd, because there is no proof that the virus is present. How do you sequence something when you haven’t shown it exists?

Readers who are unfamiliar with my work (over 300 articles on the subject of the 'pandemic' during the past year [4]) will ask: Then why are people dying? What about the huge number of cases and deaths? I have answered these and other questions in great detail. The subject of this article is: have researchers proved SARS-CoV-2 exists? The answer is no.

SOURCES:

[1] https://andrewkaufmanmd.com/

[2] https://wwwnc.cdc.gov/eid/article/26/6/20-0516_article

[3] https://www.thermofisher.com/us/en/home.html

[4] https://blog.nomorefakenews.com/category/covid/

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1. JayBee says:

April 21, 2021 at 11:43 am

The Irish government have also just confirmed that they have no info at all about that isolation having taken place.https://tapnewswire.com/2021/04/breaking-irish-government-admits-covid-19-is-a-fake-hoax-scam-which-does-not-even-exist/

2. ERIC F COPPOLINO says:

April 21, 2021 at 11:50 am

EXPLANATION BY DAVIDE BATTINI

I wrote to Battini to ask about one statement in this comment, placed in bold. This applies to all claims of isolation and purification.

"There in Toronto they did not isolate and purify anything; no purified concentrate of natural and actual intact viral virion from human host. They only have BALF [bronchoalveolar lavage fluid from a patient] supernatant [skimmed liquid] put in cell culture with animal cells, usually VeroE6 et similia, immortalized (pure poison and viral enrichers) to be redistributed to other laboratories. Then they ‘crumbled’ the RNA in the BALF in a broth of RNA of all types, and they sequenced it in RT-q-PCR…No true viral ‘purified isolate’. That is why they will never answer you." Which RNA is crumbled into the broth?

Davide sent Jan 4 2021 at 8:15 PM

Here are two ways to get patient sample.

1) IMPURE sample from BALF/SWAB/STOOL/histological/fluid directly from the patient, the Host.

2) Same IMPURE thing as point 1) but in addition then, the sample also ends up in an IMPURE cell culture (almost always cultures in monkey kidney aka VeroE6) to be amplified i.e. multiplied in culture to have the presumed viral sample in any quantity, for tests, PCR and store stock and for research and sale to labs in dedicated sites like BEI.

Both samples are still a swill of 'particles' and intact human genetic material, cells, virions and bacteria, EVs and exosomes from the Host, and so much more, (and even more came from "transfection" during replication in culture when the Host sample is cultured).

They are always IMPURE and never isolated and purified either from the human Host directly or from the supernatant after the culture.

These two types of IMPURE samples are transformed into RNA strands (double or single strands of gene nucleotides from DNA or RNA).

RNA lysates are actually crumbled RNA strands; they are cleaned/uncombed, dissolved and physiochemically filtered from their contents, i.e. cells, viruses, exosomes, etc.; all cellular and protective parts of the particles, debris, bacteria, fungi, cell bodies, and capsids and spikes around viruses present in the Host or culture supernatant sample are destroyed and dissolved, leaving only the RNA strands (whole or randomly broken up during processing).

It is called RNA purification or RNA purified, (which has nothing to do with purifying pathogen X) so now we have the RNA strands of ALL, repeat, ALL, the DNA (splitted in RNAs) and the RNA of what the IMPURE sample contained.

Now we have the crumbling of RNA strands ( or only the double strand DNA if it is PCR ) that will end up in an RT-qPCR to tell us what this test has been prepared and calibrated to tell us about these strands in the sample; or more accurately about the nucleic acid series and the genes that these RNA strands express and contain.

If we are talking about molecular testing we generally stop there.

Otherwise from this "product" of this RT-PCR or PCR we continue and proceed to the various steps of NGS genomic sequencing.

Here I stop, the whole sequencing part starts and it is boring stuff.

***

https://blog.nomorefakenews.com/2020/01/23/china-virus-epidemic-the-gong-show-on-roller-skates/

https://blog.nomorefakenews.com/2020/02/05/my-bottom-lines-on-the-china-epidemic/

https://blog.nomorefakenews.com/2020/05/21/corona-never-died-dying-now-must-be-the-virus/

https://blog.nomorefakenews.com/2020/09/03/how-many-people-have-psychological-covid/

https://blog.nomorefakenews.com/2021/01/29/covid-if-there-is-no-virus-why-are-people-dying/

***

Here’s Clif High talking about unvaxxed people catching MRNA from the vaccinated. He thinks it’s caught by breathing the skin cells which the vaxxed people are shedding 24/7: https://www.bitchute.com/video/VJ4A4yu0veDA/

This is not genocide. We are looking at the extinction of the human race….